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Facilities

ECOTOX research activities take place at the Molecular Laboratory located at CITEXVI (Lab 99, 2nd floor) and at Toralla Marine Station (ECIMAT) integrated in the European Marine Biological Research Centre (EMBRC-ERIC).

Equipment available:

Own:

  • Molecular biology:
    • DIGICEN 20R Refrigerated Centrifuge, ORTO ALRESA.
    • Molecular Imager Gel Doc™ XR+ System with Image Lab™ Software, BIO-RAD, automated image capture and analysis of gels and blots, 2 modes (trans-UV and epi-white).
    • Trans-Blot Turbo™ Transfer System, BIO-RAD: western blotting transfer system.
    • T100™ Thermal Cycler, BIO-RAD: nucleic acid amplification / make running PCR.
    • Mini-PROTEAN Tetra Handcast Systems, BIO-RAD: electrophoretic chamber used in polyacrylamide gel electrophoresis (PAGE or SDS-PAGE).
    • Wide Mini-Sub Cell GT Cell, BIO-RAD: electrophoretic chamber used in nucleic acid gel (Agarose).
    • PowerPac™ Basic power, BIO-RAD: power supply to horizontal and mini vertical gel electrophoresis (Nucleic acid gel electrophoresis / Protein gel electrophoresis).
  • Leica DMI 4000B inverted microscope, Digital Camera Leica DFC 295, and Image Analysis Software LAS (Leica Application Suite).
  • Polarographic Stand 663VA Methrom with Eco Chemie Autolab PGSTAT10 potentiostat and BASi potentiostat CGME with BAS CV-50W for the determination of metals in seawater.
  • BOD Sensor VELP System 6 with incubator.
  • Oximeter OXI 330i Crison; Salinometer w/ conductivity cell 013010-ATI-Orion; Metrohm 744 pH meter: water quality measurement equipments.
  • Cryopreservation system of CryoLogic CONTROL SYSTEMS Pty. Ltd. for cryopreservation of biological material.

Shared:


ecimat

ECIMAT

  • Sea water system (3000 L) to maintain stocks of marine invertebrates: Routine maintenance of adult stocks of echinoderms (Paracentrotus lividus) crustaceans (Acartia sp, Corophium sp.) or fish with the aim of obtaining biological material for embryolarval bioassays.
  • Isothermal rooms for phytoplankton culture with routine cultures Isochrysis galbana, Tetraselmis suecica, Chaetoceros gracilis, Selenastrum capricornutum and other microalgal species used in bioassays.
  • Nikon Microscopes: 90i fluorescence microscope, inverted microscope TE2000S, 50i Microscopes, Microscope Inverted Microscope Eclipse TS100 and E200.
  • Nikon Magnifying: SMZ1500, SMZ1000, SMZ70 and SMZ800.
  • Isothermal Rooms for embryolarval bioassays.
  • Freezers at -20 °C and -80 °C.
  • Stoves 80, INDELAB IDL-EI-208.
  • Muffle Oven, Obersal 10 PR/400 PAD.
  • Autoclave Raypa AES110.
  • DirectQ-5, ultrapure water.
  • Refrigerated Centrifuge Hettich Zentrifugen Rotanta 460 R.
  • Beckman DU640 spectrophotometer.
  • Freeze Dryer Christ Alpha 1-4.
  • Particle Counter, Beckman Coulter Multisizer 3.
  • Gas flumes.
  • PH meter Crison GLR 21
  • Oxyguard Handy Polaris
  • Portable Oxymeter, Oxymeter YSI 500, Thermo-salinity meter Radiometer PIONEER 30.
  • Membrane filtration system 0.22 mm, Millipore YT30 142 HW.
  • Distiller Raypa DES 4.
  • Magnetic stirrers, precision balances.

 

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