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Facilities

ECOTOX research activities take place at molecular laboratory located at CITEXVI (University of Vigo) and Toralla Marine Station-ECIMAT (University of Vigo) integrated in the European Marine Biological Research Centre (EMBRC-ERIC).

Equipment available:

Own:

  • Molecular biology:
    • DIGICEN 20R Refrigerated Centrifuge, ORTO ALRESA.
    • Molecular Imager Gel Doc™ XR+ System with Image Lab™ Software, BIO-RAD.
    • Automated image capture and analysis of gels and blots.
    • 2 modes (trans-UV and epi-white).
    • Trans-Blot Turbo™ Transfer System, BIO-RAD: western blotting transfer system.
    • T100™ Thermal Cycler, BIO-RAD: nucleic acid amplification / make running PCR.
    • Mini-PROTEAN Tetra Handcast Systems, BIO-RAD: electrophoretic chamber used in polyacrylamide gel electrophoresis (PAGE or SDS-PAGE).
    • Wide Mini-Sub Cell GT Cell, BIO-RAD: electrophoretic chamber used in nucleic acid gel (Agarose).
    • PowerPac™ Basic power, BIO-RAD: power supply to horizontal and mini vertical gel electrophoresis (Nucleic acid gel electrophoresis / Protein gel electrophoresis).
  • Leica Inverted microscope, Digital Camera Leica DFC 295, and Image Analysis Software LAS (Leica Application Suite).
  • Polarographic Stand 663VA Methrom with Eco Chemie Autolab PGSTAT10 potentiostat and BASi potentiostat CGME with BAS CV-50W for the determination of metals in seawater.
  • BOD Sensor VELP System 6 with incubator.Oximeter OXI 330i Crison; Salinometer w/ conductivity cell 013010-ATI-Orion; Metrohm 744 pH meter: water quality measurement equipments.
  • Cryopreservation system of CryoLogic CONTROL SYSTEMS Pty. Ltd. for regulating the temperature during the cryopreservation of biological material.

Shared:

ecimat
ECIMAT

  • Sea water system (3000 L) to maintain stocks of marine invertebrates: Routine maintenance of adult stocks of echinoderms (Paracentrotus lividus) crustaceans (Acartia sp, Corophium sp.) or fish with the aim of obtaining biological material for embryolarval bioassays.
  • Isothermal rooms for phytoplankton culture with routine cultures Isochrysis galbana, Tetraselmis suecica, Chaetoceros gracilis, Selenastrum capricornutum and other microalgal species used in bioassays.
  • Nikon Microscopes: 90i fluorescence microscope, inverted microscope TE2000S, 50i Microscopes, Microscope Inverted Microscope Eclipse TS100 and E200.
  • Nikon Magnifying: SMZ1500, SMZ1000, SMZ70 and SMZ800.
  • Isothermal Rooms for embryolarval bioassays.
  • Freezers at -20 °C and -80 °C.
  • Stoves 80, INDELAB IDL-EI-208.
  • Muffle Oven, Obersal 10 PR/400 PAD.
  • Autoclave Raypa AES110.
  • DirectQ-5, ultrapure water.
  • Refrigerated Centrifuge Hettich Zentrifugen Rotanta 460 R.
  • Beckman DU640 spectrophotometer.
  • Freeze Dryer Christ Alpha 1-4.
  • Particle Counter, Beckman Coulter Multisizer 3.
  • Gas flumes.
  • PH meter Crison GLR 21
  • Oxyguard Handy Polaris
  • Portable Oxymeter, Oxymeter YSI 500, Thermo-salinity meter Radiometer PIONEER 30.
  • Membrane filtration system 0.22 mm, Millipore YT30 142 HW.
  • Distiller Raypa DES 4.
  • Magnetic stirrers, precision balances.

 

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